DNA-transcription factor interactions: binding sites recognition by crystallography and single molecule studies

Tuesday 29 May 2018, 14:00 → 14:45 Europe/Stockholm

MAX III, Building E (MAX IV)

Jie Nan (MX-group)

Xiao-Dong Su

Professor
President of Chinese Crystallographic Society (CCrS);
Vice-president of Asian Crystallographic Association (AsCA)
Deputy director, Biodynamic Optical Imaging Center (BIOPIC)
School of Life Sciences, Peking University
100871 Beijing, China
Email: xdsu@pku.edu.cn

Abstract

Specific and non-specific protein-DNA interactions leading to genetic and epi-genetic effects are among the most fundamental processes in biology, yet the detailed mechanisms governing these interactions are not clearly understood. Combined with biochemical, crystallographic, and single molecule biophysical methods we are trying to study these processes with some model systems. We have chosen three human transcription factors (TFs): glucocorticoid receptor (GR), brain and muscle ARNT-like 1 (BMAL1) - circadian locomotor output cycles kaput (CLOCK) and estrogen receptor (ER) with methylated or unmethylated DNA binding sequences, using single-molecule and isothermal titration calorimetry assays, some recent results obtained in my lab and their implications will be discussed.