Workshop Program
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Monday, 14 September
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SSX Ecosystem
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11:30–13:00 Registration and Lunch
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13:00–13:15 Welcome and Workshop Introduction
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Session I – Infrastructures for Protein Crystallography (Part 1)
Chair: tba
13:20-13:50 Preben Morth, DTU Bioengineering
Catalyse4X, A national facility providing easy access to rapid experimental structural biology.
13:50-14:20 Martin Moche, Karolinska Institutet, Protein Science Facility
14:20-14:50 Coffee break
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Session II – Serial Crystallography at MicroMAX
Chair: tba
14:50-15:10 Dean Lang, Ambient experiment capabilities at MicroMAX
15:10-15:30 Jack Stubbs, Micro-crystallization
15:30-15:50 Monika Bjecic, SX sample preparation and delivery
15:50-16:10 Cecilia Casadei, Introduction to SX data processing including real-time indicators
16:10-16:30 Participants' flash presentations (2–3 minutes each)
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Session III - MAV IV tour
16:30-17:00
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Tuesday, 15 September
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Hands-on SSX Data Collection at MicroMAX
09:00–12:00 Practical Session I - Sample preparation and data collection
10:20–10:40 Coffee break
12:00–13:00 Lunch
13:00–16:30 Practical Session II - Sample preparation and data collection
14:50–15:10 Coffee break
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Wednesday, 16 September
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Session I - Infrastructure for Protein Crystallography (Part 2)
09:00-09:30 Wolfgang Knecht
Lund Protein Production Platform (LP3) /Protein Production Sweden (PPS)
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Session II - Hands-on SSX Data processing at MicroMAX
09:30-09:50 Cecilia Casadei
Lecture 1: SX data processing workflows: from detector frames to integrated intensities, optimising parameters and geometry.
09:50-10:20 Cecilia Casadei
Lecture 2: Data reduction, data quality metrics, use of automatised workflows to MR-phasing and basic model refinement, and isomorphous difference map / extrapolated map calculation.
10:20-10:50 Coffee break
10:50-11:50 Practical tutorials
• Tutorial 1 (optional): run MAX IV in-house developed workflows for data reduction.
• Tutorial 2: Apply downstream data processing as described in Lecture 2 on a SX dataset from MicroMAX.
• Examine data from samples brought by participants.11.50-12:00 Closing discussion and feedback
12:00-13:00 Packed lunch and departure
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