Science@FELs 2018

Session

Bioscience

27 Jun 2018, 11:30

Oskar Klein Auditorium (Stockholm, Alba Nova)

Conveners

Bioscience

• Jonas Sellberg (KTH Royal Institute of Technology)

Bioscience

• Jonas Sellberg (KTH Royal Institute of Technology)

119. Taking Snapshots of Water Oxidation Reaction in Photosystem II at X-ray Free Electron Lasers

Junko Yano

27/06/2018, 11:30

Invited oral

The development of XFELs has opened up opportunities for studying the dynamics of catalysis and biological enzymes. Intense XFEL pulses enable us to apply both X-ray diffraction and X-ray spectroscopic techniques to dilute systems or small protein crystals. By taking advantage of ultra-bright femtosecond X-ray pulses, one can also collect the data under functional conditions of temperature and...

66. The SPB/SFX Instrument of the European XFEL: Commissioning outcomes and first user results

Adrian Mancuso (European XFEL)

27/06/2018, 12:00

Invited oral

First lasing at the European XFEL was demonstrated in May last year (2017). From these first photons, remarkable progress has been made in delivering beam to the instruments and conducting first user experiments only four months later in September of the same year. I will present an overview of the Single Particles, clusters and Biomolecules and Serial Femtosecond Crystallography (SPB/SFX)...

69. Capturing reaction intermediates of enzymes by time-resolved XFEL crystallography

Minoru Kubo (RIKEN SPring-8 Center)

27/06/2018, 13:30

Invited oral

Time-resolved (TR) crystallography using X-ray free electron lasers (XFELs) is being established and increasingly applied to proteins for visualizing their structural dynamics as "molecular movies". At SACLA, we have applied this technique to study the dynamics of two respiratory enzymes, bovine cytochrome c oxidase (CcO) and fungal NO reductase (P450nor). CcO is the terminal oxidase of cell...

107. Observations of ultrafast light-induced processes in proteins using time-resolved serial femtosecond crystallography

Gabriela Nass Kovacs (Max Planck Institute for Medical Research, Heidelberg, Germany)

27/06/2018, 14:00

Invited oral

XFELs with their ultrashort and highly intense pulses open the sub-ps time domain for time-resolved crystallography using small crystals that can be efficiently photolyzed. This is particularly appealing for the study of photosensitive proteins, which contain a light-absorbing chromophore that allows exploitation of light energy as a resource or as a carrier of information initiating intra- or...